Our resource paper on gastruloid development is finally out!! An amazing work of current and former lab members which made this possible, huge congrats to all involved!
Below you can see a shortened version of the abstract for more information:
“Gastruloids are 3D structures generated from pluripotent stem cells recapitulating fundamental principles of embryonic pattern formation. Using single-cell genomic analysis, high throughput imaging and a phenotypic compound screening we provide a resource functionally mapping cell states and types with spatial resolution. We report an early spatial variability in pluripotency exit determining a binary response to Wnt activation. Although cells in the gastruloid-core revert to pluripotency, peripheral cells become primitive streak-like. These two populations subsequently break radial symmetry and initiate axial elongation. Finally, using a dual Wnt modulation, we improve the formation of anterior structures in the existing gastruloid model. This work provides a resource to understand how gastruloids develop and generate complex patterns in vitro.”
Suppinger, Zinner, Aizarani, Lukonin et al., Cell Stem Cell (2023)